rabbit igg anti human tjp1 zo1  (Proteintech)

Journal: PLoS ONE

Article Title: CD46 knock-out using CRISPR/Cas9 editing of hTERT immortalized human cells modulates complement activation

doi: 10.1371/journal.pone.0214514

Figure Lengend Snippet: a Immunofluorescence of E-cadherin and ZO1. b Gamma-glutamyltransferase activity. c FACS analysis of CD13 (Aminopeptidase N). D: Dome formation.

Article Snippet: Staining was done overnight at 4°C using goat anti-human E-Cadherin antibody (R&D Systems) diluted 1:100 and rabbit anti-human ZO1 antibody (Thermo Scientific) diluted 1:100.

Techniques: Immunofluorescence, Activity Assay

Journal: Cell transplantation

Article Title: Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell-Replacement Therapy

doi: 10.3727/096368915X688948

Figure Lengend Snippet: Quantitative PCR Oligonucleotides

Article Snippet: Detection of the RAB7 protein, a housekeeping gene that regulates vesicular transport, was used as a loading control ( 11 ). table ft1 table-wrap mode="anchored" t5 caption a7 Target Protein Isotype Immunogen Species Clonality Dilution Vendor Catalog Number Primary Antibodies AQP1 rabbit IgG human polyclonal 1:1000 Millipore AB3272 ATP1A1 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 3010 CD200 Goat IgG human polyclonal 1:1000 R&D Systems AF2724 CDH2 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 4061 COL8A2 rabbit IgG human polyclonal 1:1000 Santa Cruz Biotechnology sc-82843 GPC4 mouse IgG1 K human monoclonal 1:500 Novus Biologicals NBP1-45286 RAB7 rabbit IgG human monoclonal 1:1000 Cell Signaling Technology 9367 SLC4A11 rabbit IgG human polyclonal 1:1000 Abcam Ab93109 TCF4 rabbit IgG human monoclonal 1:400 Cell Signaling Technology 2569 ZEB1 rabbit IgG human monoclonal 1:500 Cell Signaling Technology 3396 ZO1 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 5406 Secondary Horseradish Peroxidase Antibodies Rb-IgG goat IgG rabbit polyclonal 1:30,000 Millipore AP132P Ms-IgG goat IgG mouse polyclonal 1:30,000 Millipore AP124P Gt-IgG donkey IgG goat polyclonal 1:30,000 Millipore AB324P Open in a separate window Antibodies Used for Immunoblotting Statistical Analyses The mean and standard error of the mean (SEM) were graphed for each of the transcript abundance values determined by RNA-seq (RPKM) and qPCR (2 −ΔC T).

Techniques: Real-time Polymerase Chain Reaction, Functional Assay

Journal: Cell transplantation

Article Title: Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell-Replacement Therapy

doi: 10.3727/096368915X688948

Figure Lengend Snippet: Antibodies Used for Immunoblotting

Article Snippet: Detection of the RAB7 protein, a housekeeping gene that regulates vesicular transport, was used as a loading control ( 11 ). table ft1 table-wrap mode="anchored" t5 caption a7 Target Protein Isotype Immunogen Species Clonality Dilution Vendor Catalog Number Primary Antibodies AQP1 rabbit IgG human polyclonal 1:1000 Millipore AB3272 ATP1A1 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 3010 CD200 Goat IgG human polyclonal 1:1000 R&D Systems AF2724 CDH2 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 4061 COL8A2 rabbit IgG human polyclonal 1:1000 Santa Cruz Biotechnology sc-82843 GPC4 mouse IgG1 K human monoclonal 1:500 Novus Biologicals NBP1-45286 RAB7 rabbit IgG human monoclonal 1:1000 Cell Signaling Technology 9367 SLC4A11 rabbit IgG human polyclonal 1:1000 Abcam Ab93109 TCF4 rabbit IgG human monoclonal 1:400 Cell Signaling Technology 2569 ZEB1 rabbit IgG human monoclonal 1:500 Cell Signaling Technology 3396 ZO1 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 5406 Secondary Horseradish Peroxidase Antibodies Rb-IgG goat IgG rabbit polyclonal 1:30,000 Millipore AP132P Ms-IgG goat IgG mouse polyclonal 1:30,000 Millipore AP124P Gt-IgG donkey IgG goat polyclonal 1:30,000 Millipore AB324P Open in a separate window Antibodies Used for Immunoblotting Statistical Analyses The mean and standard error of the mean (SEM) were graphed for each of the transcript abundance values determined by RNA-seq (RPKM) and qPCR (2 −ΔC T).

Techniques:

Journal: Cell transplantation

Article Title: Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell-Replacement Therapy

doi: 10.3727/096368915X688948

Figure Lengend Snippet: Proteins encoded by the genes associated with HCEnC function and corneal endothelial dystrophies were assessed by Western blotting. (A) Proteins from 5/6 (AQP1, ATP1A1, CDH2 and ZO1), 6/6 (AQP1, ATP1A1, CDH2, GPC4 and ZO1) and 3/6 (ATP1A1, CDH2 and ZO1) genes associated with HCEnC function were detected in evHCEnC, pHCEnC and the three HCEnC lines, respectively. (B) Proteins from 4/4 (COL8A2, SLC4A11, TCF4 and ZEB1), 3/4 (COL8A2, SLC4A11 and ZEB1), 1/4 (ZEB1) and 2/4 (COL8A2 and ZEB1) genes associated with corneal endothelial dystrophies were detected in evHCEnC, pHCEnC, HCEnC-21T and the two (HCEC-12 and HCEC-B4G12) related cell lines, respectively. These are representative blots from three independent samples for each HCEnC group (Table 1). The detection of the RAB7 protein was used as a loading control.

Article Snippet: Detection of the RAB7 protein, a housekeeping gene that regulates vesicular transport, was used as a loading control ( 11 ). table ft1 table-wrap mode="anchored" t5 caption a7 Target Protein Isotype Immunogen Species Clonality Dilution Vendor Catalog Number Primary Antibodies AQP1 rabbit IgG human polyclonal 1:1000 Millipore AB3272 ATP1A1 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 3010 CD200 Goat IgG human polyclonal 1:1000 R&D Systems AF2724 CDH2 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 4061 COL8A2 rabbit IgG human polyclonal 1:1000 Santa Cruz Biotechnology sc-82843 GPC4 mouse IgG1 K human monoclonal 1:500 Novus Biologicals NBP1-45286 RAB7 rabbit IgG human monoclonal 1:1000 Cell Signaling Technology 9367 SLC4A11 rabbit IgG human polyclonal 1:1000 Abcam Ab93109 TCF4 rabbit IgG human monoclonal 1:400 Cell Signaling Technology 2569 ZEB1 rabbit IgG human monoclonal 1:500 Cell Signaling Technology 3396 ZO1 rabbit IgG human polyclonal 1:1000 Cell Signaling Technology 5406 Secondary Horseradish Peroxidase Antibodies Rb-IgG goat IgG rabbit polyclonal 1:30,000 Millipore AP132P Ms-IgG goat IgG mouse polyclonal 1:30,000 Millipore AP124P Gt-IgG donkey IgG goat polyclonal 1:30,000 Millipore AB324P Open in a separate window Antibodies Used for Immunoblotting Statistical Analyses The mean and standard error of the mean (SEM) were graphed for each of the transcript abundance values determined by RNA-seq (RPKM) and qPCR (2 −ΔC T).

Techniques: Western Blot, Control